pcDNA3.1/Myc-His C

文章作者:[db:作者] | 2018-11-26 05:47
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  LOCUS pcDNA3.1(+)/myc-HisC 5490 bp DNA circular SYN 2-SEP-2003

  SOURCE

  ORGANISM

  COMMENT This file is created by Vector NTI

  http://www.biofeng.com/

  COMMENT VNTAUTHORNAME|biofeng.com|

  FEATURES Location/Qualifiers

  source 1..5490

  /invitrogen="128"

  /vntifkey="98"

  CDS 1038..1055

  /invitrogen="1010000"

  /vntifkey="4"

  /label=6xHis

  CDS complement(4494..5354)

  /invitrogen="1020000"

  /vntifkey="4"

  /label=Amp(R)

  polyA_site 1084..1308

  /invitrogen="1870000"

  /vntifkey="26"

  /label=BGH\pA

  primer complement(1078..1095)

  /invitrogen="1910000"

  /vntifkey="27"

  /label=BGH\reverse\primer

  promoter complement(5355..5453)

  /invitrogen="2330000"

  /vntifkey="30"

  /label=bla\promoter

  primer 769..789

  /invitrogen="1930000"

  /vntifkey="27"

  /label=CMV\forward\primer

  promoter 232..819

  /invitrogen="2180000"

  /vntifkey="29"

  /label=CMV\promoter

  CDS 993..1022

  /invitrogen="1090000"

  /vntifkey="4"

  /label=c-myc\epitope

  rep_origin 1354..1782

  /invitrogen="2500000"

  /vntifkey="33"

  /label=f1\origin

  misc_feature 902..995

  /invitrogen="1470000"

  /vntifkey="21"

  /label=MCS

  CDS 2193..2987

  /invitrogen="1260000"

  /vntifkey="4"

  /label=Neo(R)

  rep_origin complement(3676..4349)

  /invitrogen="2540000"

  /vntifkey="33"

  /label=pUC\origin

  promoter 1787..2157

  /invitrogen="2290000"

  /vntifkey="29"

  /label=SV40\early\promoter

  polyA_signal 3163..3293

  /invitrogen="1840000"

  /vntifkey="25"

  /label=SV40\pA

  primer 863..882

  /invitrogen="2100000"

  /vntifkey="27"

  /label=T7\primer

  promoter 863..879

  /invitrogen="2370000"

  /vntifkey="30"

  /label=T7\promoter

  BASE COUNT 1273 a 1427 c 1397 g 1393 t

  ORIGIN

  1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg

  61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg TAG: [db:TAG标签]

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